Analysis of the Molecular Weight of Peptides in Thymosin by Tricine-SDS-PAGE
10.3969/j.issn.1671-7414.2016.01.038
- VernacularTitle:改良的 Tricine-SDS-PAGE 电泳检测胸腺肽分子量
- Author:
Yanke CHEN
;
Xiaogong WANG
;
Yapeng WANG
- Publication Type:Journal Article
- Keywords:
thymosin;
molecular weight;
Tricine-SDS-PAGE;
thymosinα1
- From:
Journal of Modern Laboratory Medicine
2016;(1):132-133,137
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a simple and feasible method in analyzing the molecular weight distribution of peptides in thymosin.Methods Improved Tricine-SDS-PAGE electrophoresis was applied to detect the molecular weight of peptides and the content of thymosinα1 in thymosin.Results Tested the thymosin preparations on the domestic market using this im-proved method.It was demonstrated that the peptide molecular weight distribution in thymosin preparations was between 3.5~8.5 kD,also could detect the concentration of 1 μg thymosinα1 in thymosin by using this improved method.Conclusion This improved method is suitable for the analysis of peptides molecular weight distribution and the concentration of thy-mosinα1,so it can be used for control quality of thymosin preparations.
