Comparation of Two Different Methods for Rotavirus and Adenovirus Antigens Detection
10.3969/j.issn.1671-7414.2016.01.033
- VernacularTitle:两种不同方法对轮状病毒和腺病毒抗原检测的比较
- Author:
Jie YI
;
Hongjie XIE
;
Zhipeng XU
;
Rui ZHANG
;
Yaling DOU
;
Yingchun XU
- Publication Type:Journal Article
- Keywords:
stool pretreatment system;
manual method;
rotavirus;
adneovirus
- From:
Journal of Modern Laboratory Medicine
2016;(1):115-116,120
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the detecting results of rotavirus (RV)and adenovirus (AdV)antigens using auto stool pretreatment system (machine method)and manual method.Methods A total of 100 stools collectecd from diarrear patients admitted in gastroenterology outpatient department from September 2014 to Octorber 2014 in Peking University Medical College Hospital were detected to identify RV and AdV antigens using machine method and manual method respectively,and the nucletic acids of positive samples were detected by liquid chip method to verify the results.Results The RV,AdV and co-infection antigen positive detection rate using machine method were 17.0% (17/100),25.0% (25/100)and 12.0% (12/100)respectively,whereas those using the manual method were 4.0% (4/100),13.0% (13/100)and 2.0% (2/100),re-spectively.Taking the nucletic acids detection as the golden method,the false positive detection rate of RV,AdV and co-in-fection antigen using machine method were 23.5% (4/17),20.0% (5/25)and 33.3% (4/12)respectively,whereas those u-sing the manual method were 75.0% (3/4),69.2% (9/13)and 50.0% (1/2),respectively.χ2 test for paired data for RV and AdV positive detection rate,false positive detection rate of RV and false positive detection rate of AdV using two meth-ods were statistically significant (χ2 =15.0,52.8 and 47.5,P values <0.05).Two methods for detecting RV and AdV had poor consistency (Kappa value was 0.25,Kappa values <0.4).Conclusion Machine method has much more advantage on RV and AdV positive detection rate and false positive detection rate than manual method,which is good for clinical applica-tion.
