Effects of HSP70 inhibitor on expression of iNOS induced by IFN-γ
10.3969/j.issn.1001-1978.2016.03.018
- VernacularTitle:HSP70抑制剂PFTμ对干扰素γ诱导的iNOS表达的研究
- Author:
Xiaomei YUAN
;
Han LEI
;
Yong XIA
- Publication Type:Journal Article
- Keywords:
HSP;
IFN-γ;
NO;
iNOS;
PFTμ;
ische-mia-reperfusion injury
- From:
Chinese Pharmacological Bulletin
2016;32(3):390-393,394
- CountryChina
- Language:Chinese
-
Abstract:
Aim To research the effects of HSP70 in-hibitor ( PFTμ) on the expressions of iNOS induced by IFN-γ in RAW 264. 7 cells. Methods The NO con-centration was measured by Griess Kit. The expression of interest protein was measured by Western blot and iNOS mRNA was measured by RT-PCR. Mouse cardi-ac ischemia-reperfusion ( I/R ) model was established to set up the inflammatory response. These were divid-ed into control and treated groups. The infarct size was monitored on myocardial I/R mice. Results We found that PFTμsignificantly blocked the production of NO and the expression of iNOS protein and mRNA in RAW 264. 7 cells. The mechanism may be part of the inhibition of nuclear IRF-1 protein expression. We also found that PFTμ reduced the infarct size in myocardial I/R mice ( P <0. 05 ) . Conclusion These results suggest that PFTμ down-regulates the IFN-γ-induced iNOS transcription through decreasing translocated IRF-1 protein.
