Therapeutic effect of ellagic acid on Candida albicans infect mice models and its antifungal activity in vitro
10.3969/j.issn.1001-1978.2016.03.020
- VernacularTitle:鞣花酸体外抗真菌活性及对小鼠白色念珠菌感染模型的治疗作用研究
- Author:
Zhijian LI
- Publication Type:Journal Article
- Keywords:
ellagic acid;
antifungal activity;
minimal inhibitory concentration;
C. albicans;
mice;
MDA;
SOD
- From:
Chinese Pharmacological Bulletin
2016;32(3):399-402,403
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the inhibitory effect of ellagic acid( EA) on fungal strains both in vitro and in vivo. Methods The minimal inhibitory concentration ( MIC ) was determined by the NCCLS ( M27-A2 ) standard method in vitro. The therapeutic action was studied using a Candida albicans infect mice model, the survival rate and the content of SOD, MDA in ser-um were measured, and the pathological changes of liver were observed under light microscope after HE staining. Results EA was active against three Candida strains, with MICs between 25. 0 and 75. 0 mg·L-1 . The most sensitive Candida species was C. albicans ( MIC=25. 0 mg·L-1 ) . It was inactive against Can-dida glabrata. An in vivo test demonstrated that intrap-eritoneal injection of EA(40 and 80 mg·kg-1 ) signif-icantly enhanced the pathological changes of liver, im-proved the symptom, increased the weight and the SOD activity, and decreased the MDA activity in a mice in-fection model of C. albicans. Conclusion Ellagic acid has the potential to be developed as a natural antifungal agent.
