Expression of CD163 in patients of non-small cell lung and effects of cancer cell migration and proliferation
10.3969/j.issn.1000-484X.2016.03.025
- VernacularTitle:CD163分子在非小细胞肺癌患者组织中表达及其对肺癌细胞侵袭和增殖的影响
- Author:
Dongyun HUANG
;
Wenjing XU
;
Rui ZHOU
;
Xingxiang XU
;
Xudong ZHANG
- Publication Type:Journal Article
- Keywords:
CD163;
NSCLC;
Migration;
Proliferation
- From:
Chinese Journal of Immunology
2016;32(3):410-413,416
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of CD 163 in the plasma and tumor tissue of non-small cell lung cancer (NSCLC)patients,and the effects of cancer cell migration and proliferation .Methods:35 cases of NSCLC patients in our hospital as the study group,and 35 healthy volunteers as control group ,we used Real-time RCR to detect the expression of CD163 in the plasma and tumor tissue of NSCLC patients.Using small interfering RNA (siRNA) to inhibit the expression of CD163 in MH-2,and co-culture with A549.The level of cancer cell migration was observed by transwell and the level of cell proliferation was observed by CCK -8.Results:The expression of CD163 in the plasma and tumor tissue of NSCLC patients was significantly higher than the control group , the difference was statistically significant (P<0.05);after the expression of CD163 was suppressed in MH-2,the levels of cancer cell migration and proliferation were significantly decreased , the difference was statistically significant ( P<0.05 ) .Conclusion: The expression of CD163 in the plasma and tumor tissue of NSCLC patients is increased ,and may elevate the levels of cancer cell migration and proliferation.
