Determination of Luteolin-7-O-glucoside in Sedum Sarmentosum Bunge from Different Regions by HPLC
- VernacularTitle:HPLC法测定不同产地垂盆草药材中木犀草苷的含量
- Author:
Yan LIU
;
Caixia LIU
;
Yuren XING
- Publication Type:Journal Article
- Keywords:
HPLC;
Sedum Sarmentosum Bunge;
Luteolin-7-O-glucoside
- From:
China Pharmacist
2016;19(3):584-585,586
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an HPLC method for the determination of luteolin-7-O-glucoside in Sedum Sarmentosum Bunge. Methods:The chromatographic column was Waters SymmetryShield C18(250 mm ×4.6 mm,5μm). The mobile phase consis-ted of tetrahydrofuran-methanol-water-phosphoric acid (9 ∶17 ∶74 ∶0. 25). The flow rate was 1. 0 ml·min-1. The detection wave-length was 350 nm. The chromatographic column temperature was 35℃. The injection volume was 10 μl. Results: The calibration curves were linear within the range of 5. 2-208. 0 μg · ml-1 ( r =0. 999 9 ) for luteolin-7-O-glucoside. The average recovery was 99. 12%(RSD=0. 94%,n=6). Conclusion:The method is simple, rapid, accurate, specific and repeatable. It can be used for the determination of luteolin-7-O-glucoside in Sedum Sarmentosum Bunge.
