Value of real-time fluorescent quantitative polymerase chain reaction in detecting expression of miR-100 in patients with esophageal cancer
10.3969/j.issn.1673-4130.2016.06.008
- VernacularTitle:实时荧光定量聚合酶链反应检测食管癌患者血清 miR-100表达的意义
- Author:
Xiuying SHI
;
Qi WANG
;
Yanyu JIANG
;
Lin XU
;
Jie WU
;
Chen ZHANG
;
Jie YUAN
;
Shaoqing JU
- Publication Type:Journal Article
- Keywords:
esophageal cancer;
miR-100;
real-time fluorescent quantitative polymerase chain reaction
- From:
International Journal of Laboratory Medicine
2016;37(6):738-739,742
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the expression of serum miR-100 in patients with esophageal cancer and healthy person ,and explore the value of miR-100 in diagnosis for esophageal cancer .Methods Real-time fluorescent quantitative polymerase chain reac-tion was used to detecting miR-100 in 40 esophageal cancer patients(study group) and 50 healthy person(control group) .Results The expression of miR-100 in the study group and control group were 6 .399 ± 3 .541 ,2 .625 ± 1 .515 respective ,the expression in the study group was significant higher than that of the control group(t= 9 .07 ,P< 0 .05) .The under area of receiver operating char-acteristic curve of miR-100 in diagnosis for esophageal cancer was 0 .832(95% confidence interval was 0 .731 - 0 .934) ,when the Cut off value was 5 .285 ,the sensitivity and specificity of miR-100 in diagnosis for esophageal cancer were 65% and 95% . Conclusion Serum miR-100 in esophageal cancer patients is higher than that in healthy person ,which might be a new molecular markers in diagnosis for esophageal caner .
