Protective effect of Panax Notoginseng Saponins of Nrf2 signaling pathway on apoptosis of PC12 cells induced by Aβ25-35
10.3969/j.issn.1001-1978.2016.03.010
- VernacularTitle:基于Nrf2信号通路的三七总皂苷对Aβ25-35诱导PC12细胞凋亡的保护作用机制研究
- Author:
Jingyu ZHAO
;
Mengxia WANG
;
Ziming ZHAO
;
Xiangbao MENG
;
Guibo SUN
;
Xiaobo SUN
- Publication Type:Journal Article
- Keywords:
Alzheimer ’ s disease;
panax notoginseng saponins;
oxidative stress;
apoptosis;
Nrf2;
HO-1
- From:
Chinese Pharmacological Bulletin
2016;32(3):343-348,349
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the protective effect of Panax Notoginseng Saponins ( PNS ) on Aβ25-35-in-duced apoptosis in PC12 cells and molecular mecha-nism. Methods The cell viability of PC12 cells was detected by MTT assay. The levels of LDH leakage, ROS,MDA,Caspase-3 activity and antioxidant enzyme activities of SOD, CAT, and GSH-Px activity were de-termined by respective kits. The apoptosis of cells was decteced by Western blot. Results PNS could signifi-cantly inhibit the decrease of cell viability and LDH leakage, reduce the production of MDA and ROS( P<0. 01), increase the activity of SOD,CAT and GSH-Px ( P <0. 01 ) , and the mitochondrial membrane poten-tial, inhibit the activation of caspase-3 ( P <0. 01 ) in PC12 cells which were induced by Aβ25-35 . PNS could incrase nuclear Nrf2 and up-regulate HO-1 . The neu-roprotective of PNS could be inhibited by HO-1 inhibi-tor ZnPP. Conclusion PNS may inhibit Aβ25-35-in-duced oxidative stress and apoptosis in PC12 cells by activating Nrf2/HO-1 signaling pathway.
