Content Determination of Sinomenine in Sinomenine External Applied Powder by UPLC
10.3969/j.issn.1005-5304.2016.03.024
- VernacularTitle:超高效液相色谱法测定青藤散中青藤碱含量
- Author:
Rui WANG
;
Chenxue LI
;
Yue HU
;
Yuyan GUO
;
Yongji LI
- Publication Type:Journal Article
- Keywords:
Sinomenine External Applied Powder;
sinomenine;
UPLC;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(3):89-90,91
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an UPLC method for the determination of sinomenine in Sinomenine External Applied Powder. Methods The UPLC method was carried out on a C18 column by using acetonitrile-water-ethylene diamine (50:50:0.25) as mobile phase. The flow rate was 0.2 mL/min; the sample quantity was 2 μL; the detection wavelength was 283 nm. Results The peak time was within 1 min or so. The calibration curve of sinomenine was in the linear range of 34.2–2188.0 ng. Conclusion The method is simple, rapid, stable and reliable, which can be used for the determination of sinomenine in Sinomenine External Applied Powder.
