Extraction Technology Optimization of Total Flavonoids in Geum Aleppicum by Box-Behnken Design and Response Surface Methodology
- VernacularTitle:Box-Behnken设计响应面法优选水杨梅总黄酮提取工艺研究
- Author:
Cheng CHEN
;
Suomin FENG
;
Guoping LUO
;
Cunlao ZHANG
;
Meng DU
- Publication Type:Journal Article
- Keywords:
Geum aleppicum;
Box-Behnken design;
Total flavonoids;
Extraction
- From:
China Pharmacist
2016;19(2):241-243,250
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze and optimize the extraction technology of Geum aleppicum by Box-Behnken design and response surface methodology. Methods:With the content of total flavonoids as the index, the effects of three factors including the solvent-solid ratio, ethanol concentration and extraction time on the content of total flavonoids were studied by single factor experiment, and the ex-traction technology was optimized by Box-Behnken design and response surface methodology. Results: The optimal extraction condi-tions of Geum aleppicum were as follows:the solvent-solid ratio was 4. 2, the ethanol concentration was 50% and the extraction time was 80 min. Under the above conditions, the average yield was 12. 590 0 mg·g-1 . Conclusion: Optimizing extraction technology of the total flavonoids in Geum aleppicum by Box-Behnken design and response surface methodology is reasonable, and the mathematical mod-el is consistent with the experimental data, which has good predictability.
