Determination of Coenzyme A in Coenzyme Complex for Injection by HPLC
- VernacularTitle:HPLC法测定注射用复合辅酶中辅酶A的含量
- Author:
Lan XU
;
Xiaoxuan TAO
;
Chuanhua FENG
;
Lang ZHANG
;
Gang LI
- Publication Type:Journal Article
- Keywords:
Coenzyme complex for injection;
Coenzyme A;
HPLC;
Content determination
- From:
China Pharmacist
2016;19(2):390-391,392
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an HPLC method for the determination of coenzyme A in coenzyme complex for injection. Methods:The content determination was performed on an Intersil ODS-3 column with methanol-pH 6. 5 phosphate buffer solution (10∶90) as the mobile phase. The detection wavelength was 259 nm and the flow rate was 1. 0 ml·min-1 . The column temperature was 30℃ and the injection volume was 20 μl. Results:The linear range of coenzyme A was 1.624-32.482 u·ml-1(r=0.999 9). The average recovery was 102. 36% and RSD was 1. 14%(n=6). Conclusion: The method is simple, accurate and reproducible, and it can be used for the quality control of coenzyme complex for injection.
