Expression and Epigenetic Regulation of BRCA1 in Chemosensitive and Chemoresistant Ovarian Cancer
10.12007/j.issn.0258-4646.2016.05.001
- VernacularTitle:BRCA1在不同化疗敏感性浆液性卵巢癌中的表达及表观遗传学调节机制
- Author:
Da LI
;
Fangfang BI
;
Qing YANG
- Publication Type:Journal Article
- Keywords:
ovarian cancer;
BRCA1;
chemosensitivity;
epigenetic;
DNA methylation
- From:
Journal of China Medical University
2016;45(5):385-388,393
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of BRCA1 in chemosensitive and chemoresistant ovarian cancer specimens,so as to provide a novel insight into the epigenetic mechanism involved in BRCA1 transcription. Methods Serous ovarian cancer patients(10 chemosensitive and 10 chemoresistant cancer)were enrolled for the study. BRCA1 levels was analyzed by real?time quantitative PCR. The methylation levels of BRCA1 core promoter(sites 1?4)was determined by pyrosequencing. Regression analysis was used to examine the possible relationship between BRCA1 levels and the methylation levels of sites 1?4 in ovarian cancer specimens. Results Compared to chemosensitive ovarian cancer tissues,BRCA1 levels were increased,but the methylation levels of BRCA1 core promoter(sites 1?4)were decreased in chemoresistant ovarian cancer tissues. How?ever,it is interesting to note that only a significant inverse correlation was observed between BRCA1 levels and the methylated levels of site 4 (r=-0.612,P<0.05). Conclusion Our findings imply that the methylation levels of site 4 in the core promoter of BRCA1 may be widely involved in the regulation of BRCA1 expression and chemosensitivity in ovarian cancer.
