Expression and purification of Trop2 virus like particles
10.3969/j.issn.1673-4130.2016.08.002
- VernacularTitle:Trop2病毒样颗粒的制备及纯化研究
- Author:
Lin WANG
;
Xi WANG
;
Ke DONG
;
Huizhong ZHANG
- Publication Type:Journal Article
- Keywords:
Trop2;
tumor vaccine;
virus like particles
- From:
International Journal of Laboratory Medicine
2016;37(8):1020-1022,1025
- CountryChina
- Language:Chinese
-
Abstract:
Objective To gain the virus like particles (VLPs) based on Trop2 targets ,which provided the basis for further in vi‐vo induced experiments and anti‐tumor vaccine studies .Methods Using molecular cloning method to constract eukaryotic expres‐sion vector of pCAGGs/Trop2 and baculovirus expression vector of pFastbac1/Trop2 ,expression product of pCAGGs/Trop2 in HeLa cells were intended to be to be anchored to the cell membrane by the methods of Immunohistochemistry ;pFastbac1/Trop2 were transformed into E .coliDH10bac isolates to gain recombinant bacmids ,which were transfected into insect cells to express re‐combinant baculovirus with Gag rBV ,then sucrose density gradient centrifugation ,Western Blot and electron microscope were per‐formed to purify and identify the Trop2 VLPs .Results Building recombinant bacmids which were transfected into insect cells to express recombinant baculovirus with Gag rBV ,gained the recombinant Virus like Particles .Conclusion Trop2 VLPs was success‐fully prepared ,which laid the foundation for the subsequent induction of humoral and cellular immune response .
