BPOZ inhibits proliferation of breast cancer cell lines
10.7644/j.issn.1674-9960.2016.04.010
- VernacularTitle:BPOZ抑制乳腺癌细胞系增殖的初步研究
- Author:
Penghao WANG
;
Pingping ZHANG
;
Congwen WEI
;
Yanhong ZHANG
;
Shengli MA
;
Ye CAO
;
Yongjie ZHU
;
Xiang HE
;
Hui ZHONG
- Publication Type:Journal Article
- Keywords:
BTB/POZ (ABTB1);
breast neoplasms;
cell proliferation
- From:
Military Medical Sciences
2016;40(4):308-310,330
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of BTB/POZ ( broad complex, tramtrack and bric a brac/poxviruses and zinc finger) on proliferation of breast cancer cell lines.Methods The eukaryotic expression plasmid pCMV-HA-BPOZ was constructed by cloning from cDNA of human genome.Western blotting was used to detect the expression of pCMV-HA-BPOZ.Cells growth assay was used to detect the effect of BPOZ on proliferation and colony formation assay was used to detect the effect of BPOZ on cell growth ability.Results Western blotting showed that HA-BPOZ was efficiently expressed in cells.Moreover, the growth ability and proliferation of cells were significantly inhibited in BPOZ overexpressed cells compared with the control cells (both P <0.05).Conclusionp CMV-HA-BPOZ plasmid is constructed.BPOZ can restrain breast cancer cell lines MCF7 and ZR-75-1 cells from proliferating and growing.The results of our study can con-tribute to the study of functions of BPOZ in breast cancer.
