Effect of dexmedetomidine on the renal function and expression of tight junction protein ZO-1 and occludin in the kidney of rats with septicopyemia
10.3969/j.issn.1671-7856.2016.04.003
- VernacularTitle:右美托咪定对脓毒血症大鼠肾功能及紧密连接蛋白表达的影响
- Author:
Jinjie LI
;
Jilong KANG
;
Ying ZHAO
;
Ying ZHAO
- Publication Type:Journal Article
- Keywords:
Dexmedetomidine;
Septicopyemia;
Renal function;
Occludin;
ZO-1;
Kidney;
Rat
- From:
Chinese Journal of Comparative Medicine
2016;26(4):14-17,45
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of dexmedetomidine on the renal function and the expression of tight junction protein ZO-1 and occluding in the kidney of rats with septicopyemia induced by lipopolysaccharide.Methods Thirty adult male SD rats were randomly divided into 3 groups ( n=10 each):control group( group C) , LPS group ( group M) , and dexmedetomidine group ( group Dex ) .In the groups Dex and LPS, the rats were infused with saline and lipopolysaccharide (5 mg/kg, i.v.) respectively.In the Dex group, after intravenous injection of lipopolysaccharide (5 mg/kg) , the rats were firstly infused the loaded dose of dexmedetomidine (7μg/kg) for 15 minutes, and then changed to 5 μg/kg· min for 30 minutes.Blood samples were collected at 24 h later for measuring TNF-α, IL-1β, Scr and BUN.The kidney tissues were examined by histopathology.The expression of ZO-1 and occludin was detected by Western blot. Results Compared with the group C, the kidney tissue of group M was extensively damaged with tubular dilatation and inflammation, while reduced in the group Dex.Compared with the group C, the expressions of Scr, BUN, IL-1βand TNF-αwere all enhanced in the groups M and Dex ( P <0.05 ) , while the inflammatory factors in the group Dex were significantly lower than those of the group M ( P <0.05 ) .The Results The Western blot analysis showed that the expressions of protein ZO-1 and occludin in the group Dex were significantly higher than those of the group M (P<0.05). Conclusions Dexmedetomidine can improve the renal function of rats with septicopyemia, inhibit the acute renal injury and inflammation, increase the expression of protein ZO-1, and exert certain protective effect on the kidneys.
