Primary culture of rabbit fibroblast-like synoviocytes using an improved explant culture method

VernacularTitle:改良组织块法分离培养兔成纤维样滑膜细胞
Author: Fang CHEN ; Qin LIU ; Liping WANG ; Lifeng CHEN ; Yi ZHANG
Publication Type:Journal Article
Keywords: Improved explant culture method; Rabbit fibroblast-like synoviocytes; Isolation; Culture
From: Chinese Journal of Comparative Medicine 2016;26(4):75-78,85
CountryChina
Language:Chinese
Abstract: Objective To establish an easier and better method for primary culture of rabbit fibroblast-like synoviocytes(FLS) by modified tissue cell culture.Method Healthy rabbit joint synovial layers were cut into small pieces and siphoned off water attached on the tissue with sterile filter paper and then cultured.The growth status and morphology were observed.The growth curve of the 3rd passage cells was measured by CCK-8 assay, and the expression of vimentin was detected by immunocytochemistry.Results The FLS cultured in vitro exhibited a spindle-shaped appearance and could rapidly expand.The cell growth curve was typical of S type, and the cells highly expressed vimentin.Conclusions Primary culture of rabbit FLS by the improved explant culture method is successfully established.The method is simple and highly efficient.It provideds a new method for isolation of FLS.