Investigation and analysis on infection situation of rare adenovirus serotype among normal population in Shandong area
10.3969/j.issn.1673-4130.2016.07.021
- VernacularTitle:山东地区健康人群腺病毒稀有血清型感染状况调查分析
- Author:
Guoying WANG
- Publication Type:Journal Article
- Keywords:
adenovirus;
vector;
serotype;
neutralizing antibodies;
ELISA
- From:
International Journal of Laboratory Medicine
2016;37(7):923-924,927
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the infection status of different adenovirus serotypes of adenovirus among normal popula‐tion in Shandong area in order to screen out the serotypes of low prevalence level for the use in the adenoviral vector construction . Methods Serum levels of neutralizing antibodies against different serotypes of adenovirus were determined with ELISA by using different adenovirus serotype standard strains .Results The positive rate of total adenovirus antibodies in the normal population aged 18-70 years old in Shandong area was 95 .2% ,in which the antibody positive rates of Adv3 ,Adv5 ,Adv7 ,Adv11 ,Adv26 , Adv35 ,Adv48 and Adv49 were 69 .8% ,71 .0% ,61 .0% ,9 .4% ,2 .2% ,5 .8% ,0 .8% and 1 .6% ,respectively .The positive rates of Adv11 ,26 ,35 ,48 ,49 neutralizing antibodies were significantly lower than those of Adv3 ,5 ,7 serotypes(P<0 .01) .Conclusion The adenovirus total infection rate is high in Shandong area .The infection rates of different serotypes are different ,in which Adv26 ,48 , 49 serotypes have lower infection rates and can be used for constructing the adenovirus vector .
