Simultaneous Determination of Two Drugs in Compound Atorvastatin Calcium and Amlodipine Besylate Tablets by HPLC
- VernacularTitle:HPLC法同时测定复方阿托伐他汀钙苯磺酸氨氯地平片中两种药物的含量
- Author:
Qiuting GUO
;
Xiaofei ZHANG
- Publication Type:Journal Article
- Keywords:
Atorvastatin calcium;
Amlodipine besylate;
Determination;
HPLC
- From:
China Pharmacist
2016;19(3):610-612,613
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for the determination of atorvastatin and amlodipine in compound atorvastatin calcium and amlodipine besylate tablets. Methods:The determination was performed on a Gemini-NX C18 column (250 mm × 4. 6 mm, 5μm) with the mobile phase consisting of 20 mmol·L-1 potassium dihydrogen phosphate solution ( adjusting pH to 4 with phosphoric acid)-acetonitrile-methanol (30 ∶10 ∶60) at a flow rate of 1. 0 ml·min-1 . The detection wavelength was set at 240 nm and the column tem-perature was 30℃. The injection volume was 20μl. Results:The linear range of atorvastatine and amlodipine was 0. 4-40. 0μg·ml-1 and 0. 2-20. 0 μg·ml-1, respectively. The mean recovery of atorvastatin and amlodipine was 100. 6% and 99. 7%, and RSD was 0. 92% and 0. 85% (n=9), respectively. Conclusion:The method is special, stable and reliable, and can be used for the content determination of compound atorvastatin calcium and amlodipine besylate tablets.
