Expression levels of Tim and TLR gene family and its clinical significance in children with different types of mycoplasma pneumonia
10.3969/j.issn.1000-484X.2016.04.021
- VernacularTitle:不同类型支原体肺炎外周血TIM和TLR基因表达的变化及其意义
- Author:
Qing FAN
;
Ping YAN
;
Peijie LI
- Publication Type:Journal Article
- Keywords:
Mycoplasma pneumonia;
Bronchopneumonia;
Segmental pneumonia;
Genie expression;
qRT-PCR
- From:
Chinese Journal of Immunology
2016;32(4):546-549,552
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore expression levels of Tim and TLR gene family in children with different types of mycoplasma pneumonia and their clinical significance.Methods: 56 patients with mycoplasma pneumonia were randomly selected in Ji′nan Maternity and Child Care Hospital from March ,2013 to March,2014.All the patients were divided into bronchopneumonia group ( n=40) and segmental pneumonia group (n=16) according to their X-ray manifestations.Bronchopneumonia group was characterized as dot,nodular increased density shadow with or without reticular ,stripe-like interstitial lesion.Segmental pneumonia was described as segmental or lobar consolidation of lung in X-ray.Meanwhile,30 healthy children were involved in control group with matched age and gender.Peripheral venous blood of all the children in every group was collected and total RNA was extracted .The mRNA of Toll-recepter 2,4 and Tim1,3 was quantified by quantitative real-time PCR (qRT-PCR),and compared between different groups.Results:The expression of mRNA of TLR-2,4 and Tim1 significantly increases in both bronchopneumonia group and segmental pneumonia group compared to healthy control.Meanwhile,the mRNA of TLR-2,4 and Tim-1,3 was significantly elevated among segmental pneumonia group compared to healthy control.In addition,the gene expression of Tim1,3 was higher in segmental pneumonia group than broncho-pneumonia group.Conclusion:The TLR-2,4 and Tim1 gene are over expressed in both types of mycoplasma pneumonia and Tim-1,3 are highly expressed in children with severe infection in lung.The expression alteration of TLR-2,4 and Tim1,3 gene is involved in different types of mycoplasma pneumonia.
