Extraction Process Optimization of Total Flavonoids from Chloranthus Japonicus sieb by Box-Behnken Re-sponse Surface Methodology
- VernacularTitle:Box-Behnken效应面法优化银线草中总黄酮的提取工艺
- Author:
Jing SUN
;
Xiaofei ZHANG
;
Jieqiong LIU
;
Tianqian JIA
- Publication Type:Journal Article
- Keywords:
Box-behnken response surface methodology;
Chloranthus japonicus sieb;
Total flavonoids
- From:
China Pharmacist
2016;19(3):440-442,452
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To optimize the extraction process of total flavonoids from Chloranthus japonicus sieb by Box-Behnken re-sponse surface methodology. Methods:The independent variables were the ethanol concentration ( X1 ) , liquid-solid ratio ( X2 ) and extraction time ( X3 ) , the dependent variable was the extraction amount of total flavonoids ( Y) , and the extraction process was opti-mized by Box-Behnken response surface methodology. Results: The results showed that the optimal extraction conditions for total fla-vonoids from Chloranthus japonicus sieb were as follows: the ethanol concentration was 54. 8%, the liquid-solid ratio was 13. 6, and the extraction time was 2. 0 h. The verified extraction process parameters of three batches showed that the relative error between the ex-perimental values and those predicted from the regression model was -2. 34%. Conclusion:The optimal extraction process parameters are simple and more convenient with higher precision for the extraction of total flavonoids from Chloranthus japonicus sieb.
