Comparison of RP-HPLC and CMIA Method in the Determination of Valproate in Plasma of Epileptic Pa-tients
- VernacularTitle:比较HPLC和化学发光微粒子免疫法测定癫痫患儿血浆中丙戊酸的浓度
- Author:
Meihua GUO
;
Yan CHEN
;
Zhao QIAN
;
Yanyan MA
;
Xin HAI
- Publication Type:Journal Article
- Keywords:
HPLC;
CMIA;
Valproate;
Plasma concentration;
Correlation;
Consistency
- From:
China Pharmacist
2016;19(3):505-509,518
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the valproate concentration in plasma of epilepsy patients by HPLC, and compare with the re-sults of chemiluminescence microparticle immunoassay ( CMIA) to evaluate the consistency of the two methods. Methods:HPLC and CMIA was respectively applied to determine the plasma concentration of valproate in 230 epileptic patients. The correlation of the two methods was studied by Passing-Bablok regression and Bland-Altman method. Results:The regression equation of the determination re-sults of HPLC (Y) and CMIA (X) was Y=1. 069 7X+2. 338 2 (R2 =0. 969, n=230), which showed promising correlation. Bland-Altman analysis showed that the consistency of the two methods was poor, and the values of HPLC were higher. Conclusion: HPLC and CMIA used for the determination of valproate plasma concentration show good correlation. However, the consistency is poor and there is system error. In the clinical treatment, adjustment and choice should be paid more attention.
