Stem Cells Expressing Homing Receptors Could be Expanded From Cryopreserved and Unselected Cord Blood.
10.3346/jkms.2004.19.5.635
- Author:
Young Ho LEE
1
;
Jin Yeong HAN
;
Su Yeong SEO
;
Kyeong Hee KIM
;
Young Ah LEE
;
Young Seok LEE
;
Hyung Sik LEE
;
Won Joo HUR
;
Hun HAN
;
Hyuk Chan KWON
;
Jae Seok KIM
;
Hyo Jin KIM
Author Information
1. Hematopoietic Stem Cell Transplantation Program, Dong-A Cancer Center, Dong-A University College of Medicine, Busan, Korea. yhlee1@daunet.donga.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Receptors, Lymphocyte Homing;
Ex Vivo Expansion;
Cryopreservation;
Fetal Blood;
Cytokines;
Granulocyte Colong-Stimulating Factor;
Thrombopoietin;
Interleukin-6: Antigen, CD34
- MeSH:
ADP-ribosyl Cyclase/metabolism;
Antigens, CD/metabolism;
Antigens, CD34/metabolism;
Cryopreservation;
Fetal Blood/*cytology;
Humans;
Integrin alpha4beta1/metabolism;
Integrin alpha5beta1/metabolism;
Membrane Proteins;
Receptors, CXCR4/metabolism;
Receptors, Lymphocyte Homing/*metabolism;
Research Support, Non-U.S. Gov't;
Stem Cell Factor;
Stem Cells/*cytology/*metabolism;
Thrombopoietin
- From:Journal of Korean Medical Science
2004;19(5):635-639
- CountryRepublic of Korea
- Language:English
-
Abstract:
We assessed the cytokine combinations that are best for ex vivo expansion of cord blood (CB) and the increment for cell numbers of nucleated cells, as well as stem cells expressing homing receptors, by an ex vivo expansion of cryopreserved and unselected CB. Frozen leukocyte concentrates (LC) from CB were thawed and cultured at a concentration of 1x10(5)/mL in media supplemented with a combination of SCF (20 ng/mL)+TPO (50 ng/mL)+FL (50 ng/mL)+/-IL-6 (20 ng/mL)+/-G-CSF (20 ng/mL). After culturing for 14 days, the expansion folds of cell numbers were as follows: TNC 22.3+/-7.8~26.3+/-4.9, CFU-GM 4.7+/-5.1~11.7+/-2.6, CD34+CD38- cell 214.0+/-251.9~464.1+/-566.1, CD34+CXCR4+ cell 4384.5+/-1664.7~7087.2+/-4669.3, CD34+VLA4+ cell 1444.3+/-1264.0~2074.9+/-1537.0, CD34+VLA5+ cell 86.2+/-50.9~ 113.2+/-57.1. These results revealed that the number of stem cells expressing homing receptors could be increased by an ex vivo expansion of cryopreserved and unselected CB using 3 cytokines (SCF, TPO, FL) only. Further in vivo studies regarding the engraftment after expansion of the nucleated cells, as well as the stem cells expressing homing receptors will be required.
