Optimization of Determination Methods for Scopoletin from Caulis Erycibes

VernacularTitle:丁公藤东莨菪内酯含量测定方法的优化研究
Author: Cuiting LIU ; Dongcheng ZHENG ; Caiqun HE ; Jie PAN ; Ping YAN ; Ruoting ZHAN
Publication Type:Journal Article
Keywords: Caulis Erycibes/chemistry; Scopoletin/analysis; Chromatography,high performance liquid
From: Journal of Guangzhou University of Traditional Chinese Medicine 2015;32(6):1067-1070,1074
CountryChina
Language:Chinese
Abstract: Objective To optimize the high performance liquid chromatography (HPLC) method for determining the content of scopoletin from Caulis Erycibes. Methods Methanol-25% HCl ( v/v, 4 : 1) solvent was used to extract scopoletin. HPLC method was performed on Waters XBridge Shield RP18 column (4.6 mm × 250 mm, 5μm) with the mobile phase consisting of acetonitrile ( A) and 0.16% ( v/v) acetic acid ( B) solution by gradient elution. The detection wavelength was 298 nm and the flow rate was set at 1.0 mL/min. Results The linear range of scopoletin from Caulis Erycibes was 2.83-118 μg/mL, and the recovery rate was 99.47% ( sR=1.07%). Conclusion The optimized method is simple, specific and accurate, and can provide reference for content determination of scopoletin in Caulis Erycibes.