Quality Standard of Mongolian Medicine Rhaponticum uniflorum (L.) DC.

VernacularTitle:蒙药漏芦花质量标准研究
Author: Yue ZHANG ; Yangxue LI
Publication Type:Journal Article
Keywords: Rhaponticum uniflorum (L.) DC./chemistry; Chlorogenic acid/analysis; Chromatography,high performance liquid; Chromatography,thin layer
From: Journal of Guangzhou University of Traditional Chinese Medicine 2015;32(6):1071-1074,1149
CountryChina
Language:Chinese
Abstract: Objective To establish the quality standard for mongolian medicine Rhaponticum uniflorum (L.) DC. with modern analysis methods. Methods Chlorogenic acid and apigenin were identified by thin -layer chromatography (TLC). The chlorogenic acid content was determined by high performance liquid chromatograghy ( HPLC). And HPLC was performed on Kromat Universil C18 ( 4.6 mm × 250 mm, 5 μm) with methanol-water-acetic acid ( v/v/v, 15:85:0.85) as mobile phase, the detection wavelength was 326 nm, the flow rate was 1.0 mL/min, and the column temperature was 20℃. Results The samples and the reference substance shared the same color spots at the same sites. The linear range of chlorogenic acid was 0.044 66-0.446 6μg (r=0.999 8), and the average recovery rate was 99.84%. Conclusion The method is simple, accurate, and with good reproducibility, and can be used for the quality control of Rhaponticum uniflorum ( L. ) DC.