Study on Repairing Skin Defect with Pelnac Tissue Engineering Skin
10.13359/j.cnki.gzxbtcm.2015.03.032
- VernacularTitle:皮耐克组织工程皮肤修复皮肤创面的研究
- Author:
Xingye CHEN
;
Fan LI
;
Aijun LIU
- Publication Type:Journal Article
- Keywords:
Pelnac;
Tissue engineering;
Skin/pathology;
Cell culture;
Disease models,animal;
Nude mice
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2015;(3):529-532,580
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the feasibility of repairing skin defect with Pelnac tissue engineering skin and cultured epidermal stem cells ( ESCs) and fibroblasts. Methods ESCs and fibroblasts in SD rats were isolated and cultured in vitro, and then were detected by immunohistochemistry. Fibroblasts were seeded in Pelnac material to construct dermis, and ESCs were seeded on the surface of dermis to construct tissue engineering skin. Thirty BALC/c nude mice were randomly divided into groups A, B and C. After mouse back skin defect model was established, group A was transplanted with Pelnac material, group B was transplanted with compound of Pelnac and fibroblasts, and group C was transplanted with Pelnac tissue engineering skin. The wound healing was observed after operation. The wound tissues were sampled for pathological histology test on the 5th and 7th day after transplantation. Results The expression of cytokeratin 15 and β1 integrin in ESCs was positive, and the expression of laminin ( LN) and fibronectin ( FN) in fibroblasts was positive. In group C, the wound healing of nude mice was the best, characterized by the proliferation of fibroblasts, obvious new capillaries and regularly arranged epidermal cells at the transplantation sites. Conclusion Pelnac tissue engineering skin has good effects on promoting repair of skin defect of nude mice.
