Exploration of Biological Mechanism of Lingering Toxins Theory in Ulcerative Colitis
10.13359/j.cnki.gzxbtcm.2015.04.018
- VernacularTitle:溃疡性结肠炎伏毒理论的生物学机制探讨
- Author:
Beiping ZHANG
;
Zhiyu WU
- Publication Type:Journal Article
- Keywords:
Ulcerative colitis/TCD therapy;
Lingering toxins theory of TCM;
Matrix metalloproteinase-1;
Tissue inhibitor of metalloproteinase-1;
Prostaglandin E2
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2015;(4):661-665,670
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the biological mechanism of the Lingering Toxins Theory in ulcerative colitis (UC) by clinical trials. Methods Forty patients with UC at mild, moderate active stage and at remission stage were enrolled into the experiment. Twenty cases of UC patients at active stage were divided into Chinese medicine intervention group and western medicine treatment group, 10 in each group. Twenty cases of UC patients at remission stage were also evenly divided into Chinese medicine intervention group and western medicine treatment group. All patients were given conventional western medicine treatment, and additionally, Chinese medicine intervention group were given oral use of Chinese medicine decoction, the treatment course covering 8 weeks. Before and after treatment, plasma matrix metalloproteinase-1 (MMP -1), tissue inhibitor ofmetalloproteinase-1 (TIMP-1) and prostaglandin E2 (PGE2) expression levels were observed by enzyme-linked immunosorbent assay (ELISA) . Results Plasma MMP-1,TIMP-1, and PGE2 expression levels in UC patients were positively correlated with disease severity (P<0.001) . After treatment, the expression levels of MMP-1, TIMP-1, and PGE2 in active-stage patients of both groups were decreased compared with those before treatment (P<0.001) , but the difference between the two groups was insignificant ( P>0.05) . After treatment, the expression levels of MMP-1 and TIMP-1 in remission-stage patients of Chinese Medicine intervention group were decreased (P<0.001) , and the decrease was superior to that of Western medicine treatment group (P<0.05) . Difference of PGE2 expression level in remission-stage patients of both groups was insignificant ( P>0.05) . Conclusion There is a relation between plasma MMP-1, TIMP-1 expression levels and the “lingering toxins” in the field of traditional Chinese medicine. The biological mechanism of toxins-removing therapy in treating UC may be associated with the decrease of the plasma MMP-1 and TIMP-1 expression levels.
