Inhibitory Effect of Serum Containing Modified Fuzheng Yiliu Decoction on Human Colon Cancer Cell Line HT-29 and Its Mechanism
10.13359/j.cnki.gzxbtcm.2014.06.028
- VernacularTitle:加味扶正抑瘤汤含药血清对结肠癌HT－29细胞的抑制效应及其机制研究
- Author:
Wei WANG
;
Haigan YANG
;
Lijie LUO
;
Yupeng LIANG
;
Yansheng ZHENG
;
Dechang DIAO
- Publication Type:Journal Article
- Keywords:
Modified Fuzheng Yiliu Decoction/pharmacology;
Colon cancer/TCD therapy;
Cell proliferation;
Cell culture;
Gene expression regulation
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2014;(6):979-982,1001
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Chinese medicinal compound recipe ( modified Fuzheng Yiliu Decoction, MFYD) on the proliferation of colon cancer HT-29 cells and on the expression of cellular apoptosis gene, Bcl-2, and tumor-inhibiting gene, P53. Methods Colon cancer HT-29 cells were divided into serum containing MFYD groups ( treated with different concentrations of serum containing MFYD) and blank serum group. Methyl thiazolyl tetrazolium ( MTT) , real time cellular analysis ( RTCA) and reverse transcription poly merase chain reaction ( RT-PCR) were used to detect the cell proliferation and mRNA expression of Bcl-2 and P53 separately. Results The proliferation of colon cancer HT-29 cell line was inhibited by MFYD ( P<0.05) in concentration-and time-dependent manner. The Bcl-2 mRNA expression was decreased and p53 mRNA was increased markedly in HT-29 cells after co-culturing with 12% or 15% volume fraction of serum containing MFYD for 48 hours, the differences being significant compared with the blank serum group ( P<0.05). Conclusion MFYD has obvious inhibitory effect on the proliferation of colon cancer HT-29 cells, and its mechanism may be related to down-regulating the expression of Bcl-2 gene and up-regulating the expression of P53 gene.
