Regulatory Effect of Nervilia fordii Injection on Inflammatory Cytokines in Rats with Lipopolysaccharide-induced Acute Lung Injury
10.13359/j.cnki.gzxbtcm.2014.05.019
- VernacularTitle:青天葵注射液对内毒素诱导急性肺损伤大鼠炎症细胞因子调控的影响
- Author:
Yinji XU
;
Jiayang LIN
;
Yuanbin CHEN
;
Lei WU
;
Lin LIN
- Publication Type:Journal Article
- Keywords:
Nervilia fordii/pharmacology;
Acute lung injury/TCD therapy;
Inflammatory cytokines;
Cell culture;
Disease models,animal;
Rats
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2014;(5):772-775,847
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the regulatory effect of Nervilia fordii Injection ( NFI ) on inflammatory cytokines in rats with lipopolysaccharide ( LPS) -induced acute lung injury ( ALI) , and to explore its possible interfering mechanism . Methods The rats were randomly divided into normal group , model group , Shenmai Injection group, and NFI group. J774 macrophages were stimulated by LPS to establish the cell model in vitro, and in vivo ALI rat model was established by injection of LPS through the sublingual veins. Electronic microscope and enzyme-linked immunosorbent assay (ELISA) were used for observing the proliferation of J774 macrophages, the levels of supernatant inflammatory cytokines secreted by J774 cells, and the levels of serum inflammatory cytokines . Results The proliferation of LPS-induced J774 macrophages was increased , and the secretion of inflammatory cytokines was disordered. Uncontrolled inflammatory reaction occurred in the lung after the rats were administrated with intravenous injection of LPS . Both NFI and Shenmai Injection could inhibit the proliferation of J774 macrophages. NFI could also significantly inhibit the levels of supernatant and serum tumor necrosis factor (TNF) -α and interleukin 6 (IL-6) expression (P<0.05 or P<0.01), and it could increase the level of supernatant IL-10 (P<0.01) and decrease the level of serum interleukin 10 (IL-10) in rats (P<0.05), but couldn’t regulate the secretion of monocyte chemoattractant protein 1 (MCP-1) (P>0.05). Conclusion NFI has better preventive and therapeutic effect for ALI than Shenmai Injection, and its possible mechanism is related with the inflammatory regulation and lung injury relief through the suppression of excessive expression of TNF-α and IL-6 .
