Study on Anti-tumor Activities of Trichosanthin Mediated by Low Molecular Weight Protamine
10.13359/j.cnki.gzxbtcm.2014.05.021
- VernacularTitle:低分子量鱼精蛋白介导的天花粉蛋白的抗肿瘤应用
- Author:
Jianming LIANG
;
Feng ZENG
;
Yingzhi CHEN
;
Jiao TAN
;
Pengyu TAO
;
Qin XU
;
Yongzhuo HUANG
- Publication Type:Journal Article
- Keywords:
Trichosanthin;
Cell-penetrating peptide;
Gene recombinant technique;
Chemical coupling;
Tumor/TCM-WM therapy;
Cell culture
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2014;(5):780-785,854
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the anti-tumor activities of cell-penetrating peptide ( CPP) - mediated trichosanthin ( TCS) , which is a recombinant protein obtained from Radix Trichosanthis. Methods Cysteine residue was introduced to the C-terminus of TCS by protein recombinant technique, and then with the newly-formed terminal as the modification site, TCS was coupled with CPP. As a target protein, CPP-mediated TCS was isolated and purified by affinity chromatography. The expression of the target protein and its responsiveness to reducing substances were detected by using the sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The cellular uptake rate of CPP-mediated TCS was determined by using cell uptake test, and its anti-tumor activity was measured by using methyl thiazolyl tetrazolium (MTT) assay. Results The TCS-CPP compound had been successfully developed in this study, and showed certain reducing responsiveness. After modified with CPP, TCS had higher cellular uptake rate and stronger anti-tumor effect on HeLa and MCF-7 cells. Conclusion TCS modified by CPP can enhance the anti-tumor activities of TCS.
