Studies on Quality Standard of Radix Toddaliae Asiaticae
10.13359/j.cnki.gzxbtcm.2015.01.029
- VernacularTitle:飞龙掌血根的质量研究
- Author:
Lingling WEN
;
Runsheng ZHENG
;
Yaping XU
;
Zehui QIN
;
Hui XU
;
Ruoting ZHAN
;
Weiwen CHEN
- Publication Type:Journal Article
- Keywords:
Radix Toddaliae Asiaticae/chemistry;
Chloride nitidine/analysis;
Toddalolactone/analysis;
Quality standard;
Chromatography,thin layer;
Chromatography,high performance liquid
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2015;(1):136-140,146
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the quality standard of Radix Toddaliae Asiaticae. Methods Thin layer chromatography ( TLC) and high performance liquid chromatography ( HPLC) were used to identify and determine chloride nitidine and toddalolactone in Radix Toddaliae Asiaticae. The moisture and total ash contents were detected according to the methods recorded in appendix of Chinese Pharmacopeia (2010 edition) . Results Toddalolactone and chloride nitidine were detectable by TLC, the spots were clear and the dissociation was good. The established HPLC method was simple and accurate. The linear ranges of toddalolactone and chloride nitidine in Radix Toddaliae Asiaticae were 2.84~42.6 μg/mL and 25.6~385 μg/mL, and their recovery rates were 99.2 % ( RSD=1.12%) and 100 % ( RSD=0.71%) , respectively. The content of moisture was in the range of 75.8~98.9 mg/g and that of total ash was in the range of 12.4~33.6 mg/g. Conclusion The developed method is specific and accurate, and can provide useful reference for establishing quality standard of Radix Toddaliae Asiaticae.
