Effect of advanced glycosylation end products on bFGF expression of cultured rabbit Müller cells
10.3969/j.issn.1000-484X.2015.12.008
- VernacularTitle:糖基化终末产物对培养的兔视网膜Müller细胞bFGF表达的影响
- Author:
Ying LENG
;
Yuehui WANG
;
Daning ZHANG
;
Xiangjun LI
;
Dan BAI
- Publication Type:Journal Article
- Keywords:
AGEs;
Basic fibroblast growth factor;
Müller cell;
Diabetic retinoopathy
- From:
Chinese Journal of Immunology
2015;31(12):1621-1623,1632
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effects of different doses of advanced glycosylation end products ( AGEs ) on bFGF expression of cultured rabbit M üller cells in vitro.Methods:Immunocytochemistry and transmission electron microscopy methods were used identified cultured M üller cell.Immunocytochemistry method was used to semi-quantitate bFGF expression of retinal Müller cells at 640 μl/2 000 μl AGEs conditions.We observed effects of AGEs and PKC inhibitor Calphstion C on bFGF mRNA expression .Results:640 μl/2 000 μl AGEs stimulate bFGF expression of retinal Müller cell.Calphostin C inhibits bFGF mRNA increase stimulated by AGEs,and inhibition achieves strongest at concentration 50 nmol/L.Conclusion:AGEs can stimulate bFGF expression of Müller cell to exert the role of angiogenesis .bFGF mRNA expression may be regulated by activation of PKC pathway .
