Effect of ginsenoside Re on cytochrome P450 in H9 c2 cells

VernacularTitle:人参皂苷Re对H9 c2心肌细胞CYP450酶的影响
Author: Zengchun MA ; Yong XIAO ; Jiaowei ZHAO ; Yuguang WANG ; Honglin TAN ; Qiande LIANG ; Xianglin TANG ; Chengrong XIAO ; Ling YANG ; Yue GAO
Publication Type:Journal Article
Keywords: ginsenoside Re; cardiac myocyte; atrial natriuretic peptide; cytochrome 450; cytochrome 2 J3; cytochrome 4 A1
From: Chinese Pharmacological Bulletin 2016;32(4):494-497,498
CountryChina
Language:Chinese
Abstract: Aim To observe the cytochrome 450 effect of ginsenoside Re on H9c2 cells, in order to clarify the molecular mechanism of ginsenoside Re. Methods H 9 c 2 cells were separately treated with ginsenoside Re for 1, 5, 10, 50, 100 μmol·L-1 or 6, 24, 36, 48, 60 h. CYP2C11, 2J3, 4A1, 4A3, 4F4 and ANP mR-NA expressions were analyzed by Real time PCR, and CYP4 A1 , 2 J3 protein expressions were detected by Western blot. Results Compared with control group, ginsenoside Re could effectively upregulate CYP2 C11 , CYP2 J3 , ANP mRNA expression to 1. 6 , 1. 8 , 3. 2 fold, and downregulate CYP4A1, CYP4A3, CYP4F4 mRNA expression to 0. 4, 0. 15, 0. 3 fold. Ginsen-oside Re could decrease CYP4 A1 protein expression in a concentration-dependent manner, while ginsenoside Re could increase CYP2 J3 protein expression in a con-centration-dependent manner. Conclusion Ginsen-oside could regulate CYP450 enzyme and change ANP gene expression, which might be the molecular mecha-nism of ginsenoside Re.