PTEN inhibitors attenuates high glucose induced endothelial cell senescence
10.3969/j.issn.1001-1978.2016.04.015
- VernacularTitle:PTEN抑制剂对高糖诱导的内皮细胞衰老的影响
- Author:
Xiaoying LIU
;
Li WU
;
Zhou CHEN
;
Libin LIU
- Publication Type:Journal Article
- Keywords:
endothelial cell;
PTEN;
p16;
cell senes-cence;
PI3 K-Akt;
high glucose
- From:
Chinese Pharmacological Bulletin
2016;32(4):514-518,519
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study influence of PTEN inhibitors ( PIC) on high glucose induced endothelial cell senes-cence Methods ( 1 ) HUVECs were cultured in ECM medium. Cell viability was determined by CCK-8. Cells were randomly divided into control group ( C ) , high glucose group ( HG ) , high glucose +10 nmol · L-1 PIC group ( HG +10 nmol · L-1 PIC ) , high glu-cose+100 nmol · L-1 PIC group ( HG +100 nmol · L-1 PIC) . Cell apoptosis was examined by flow cytom-etry. Senescence Detection Kit was performed by SA-β-gal Cytochemical staining. The expression levels of genes and protein ( p16 and Pten ) were detected by real-time PCR and Western blot. Results The prolif-eration rate was markedly decreased in HG group com-pared with C group, and this phenomenon was reversed by PTEN inhibitors . Cell apoptosis was significantly increased in HG group compared with C group, and a-gain these changes were blocked by PIC. The expres-sion levels of genes ( PTEN) were significantly higher in HG group compared with C group. The frequency of senescent ( SA-β-Gal-positive ) cells and the expres-sion level of senescence genes ( p16 ) were significantly higher in HG group compared with C group, and these changes were blocked by PIC. Conclusion These re-sults show that PTEN inhibitors ( PIC ) delays cellular senescence that is promoted under high glucose condi-tion.
