Study on the Whitening activity and pathway of red eucommia herbs
- VernacularTitle:红杜仲的美白活性及其途径研究
- Author:
Jinbin LIN
;
Tong REN
;
Yijiang LIAN
;
Weijie LI
;
Xin JIN
;
Zhibo XIA
;
Wenjun LI
- Publication Type:Journal Article
- Keywords:
red eucommia herbs;
B16 cells;
melanin synthesis;
inhibition effect
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;(12):21-23,28
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the impact of PEE with different ethanol concentration extract on the activity of tyrosinase in B16 melanoma and to explore the feasibility of PEE used as skin whitening agent make use of a model organism .Methods 30%, 50%, 70%, 95%, different concentration of ethanol reflux got four kinds of PEE alcohol extract, respectively.And selected the most active inhibition to mushroom tyrosinase ( PEE30、PEE50、PEE70、PEE95).The effect of extracts from the selected on B16 cell model was determined by MTS, NaOH splitting decomposition, L-DOPA oxidation progress.The change in protein expression level after ethanol extract of PEE was determined by Western bolt.Results The capacity of inhibition to mushroom tyrosinase in each concentration of PEE extraction, but PEE70 had the most active inhibition.When challenged with B16 cell model, PEE70 showed the capacity of decrease in tyrosinase activities and melanin synthesis (P<0.01) and had a does dependence ;it also decreased Tyrosinase and TRP-1 expression compared with control B16 cells.Conclusion These data support the idea that PEE can restrain melanogenesis, through its inhibitory effect on the activity of tyrosinase.
