Regulatory effects of the bile acid membrane receptor TGR5 on FN and TGF-β1 in rat glomerular mesangial cells cultured under high glucose condition
10.3969/j.issn.1001-1978.2016.01.008
- VernacularTitle:胆汁酸膜受体 TGR5对高糖培养的大鼠肾小球系膜细胞 FN、TGF-β1的调控作用
- Author:
Fengxiao XIONG
;
Zhiying YANG
;
Shaogui WANG
;
Cheng CHEN
;
Heqing HUANG
- Publication Type:Journal Article
- Keywords:
G protein-coupled receptor;
TGR5;
high glucose;
glomerular mesangial cells;
fibronectin;
transfor-ming growth factor-β1;
INT-777;
diabetic nephropathy
- From:
Chinese Pharmacological Bulletin
2016;(1):33-36,37
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the expression of G protein-coupled receptor TGR5 and its effects on FN and TGF-β1 expression cultured under high glucose condition in rat glomerular mesangial cells , and then to explore the role of TGR5 in diabetic nephropathy. Methods INT-777 and TGR5 plasmid were used to activate TGR5 under high glucose(HG,30 mmol·L - 1 glucose ) condition, and anti-TGR5 small interfering RNA(TGR5 siRNA) was used to knock down TGR5. The protein expression of FN and TGF-β1 in rat me-sangial cells was detected by Western blot. Results TGR5 could be detected in rat glomerular mesangial cells. Both FN and TGF-β1 protein levels could be in-creased by high glucose compared with control group(P < 0. 05),and be inhibited by activiation of TGR5(P <0. 05). On the other hand,knockdown of TGR5 could increase FN and TGF-β1 protein to abnormal levels(P< 0. 01,P < 0. 05). Conclusion TGR5 suppresses HG-induced FN and TGF-β1 expression in rat glomer-ular mesangial cells,suggesting a protective role in the process of diabetic nephropathy.
