Effects of Sorting nexin 10 deficiency on function of mouse macrophages
10.3969/j.issn.1001-1978.2016.01.018
- VernacularTitle:Sorting nexin 10缺失对小鼠巨噬细胞功能的影响
- Author:
Wanzhen LI
;
Yan YOU
;
Jin PENG
;
Chun ZHOU
;
Dong LI
;
Xiaoyan SHEN
- Publication Type:Journal Article
- Keywords:
SNX10;
macrophage;
phagocytosis;
ster-ilization;
TNF-α;
IL-12 / 23p40;
IL-6;
NF-κB
- From:
Chinese Pharmacological Bulletin
2016;(1):84-88,89
- CountryChina
- Language:Chinese
-
Abstract:
Aim To discuss the influence of endosome/ lysosome transport proteins SNX10 on macrophage, providing new potential targets for the treatment of variety of related immune diseases . Methods The genotype of mice was identified by PCR. The role of SNX10 in phagocytosis of bacterial components and sterilization by macrophages were assessed. The levels of TNF-α、IL-12 / 23 p40 and IL-6 were measured by q-PCR and ELISA assay. Finally, the NF-κB signaling pathway was evaluated by Western blot and immuno-fluorescence staining. Results Ex vivo experiments showed that SNX10 knockout could enhance bactericid-al ability and inhibit the expression and production of TNF-α, IL-12 / 23 p40 and IL-6 of macrophages.These effects might attribute to the inhibition of NF-κB signaling pathway activation. Conclusion SNX10 knockout could enhance bactericidal ability and inhibit the inflammatory response of macrophages, and its mechanism may be achieved through the NF-κB signa-ling pathway.
