Protective Effect of Omega-3 Fish Oil Fat Emulsion on Cyclophosphamide-induced Gastric Mucosal Injury in Mice
- VernacularTitle:ω-3鱼油脂肪乳对环磷酰胺所致小鼠胃黏膜损伤的保护作用
- Author:
Linxiu HE
;
Mingli SUN
;
Jing BIAN
;
Zhaojin YU
;
Wenjie CHEN
;
Yanan LI
;
Minjie WEI
- Publication Type:Journal Article
- Keywords:
omega-3 fish oil fat emulsion;
cyclophosphamide;
gastric mucosal lesion
- From:
Journal of China Medical University
2015;(12):1090-1093,1115
- CountryChina
- Language:Chinese
-
Abstract:
Objective to investigate the protective effect of omega-3 fish oil fat emulsion on cyclophosphamide-induced gastric mucosal injury in mice. Methods Forty-five kunming mice were randomly divided into three groups as control,model,and omega-3 fish oil fat emulsion group(with 15 mice in each group). Mice of the two experiment groups were administrated with cyclophosphamide i.p. for 2 days to establish the damage model. then mice in omega-3 fish oil fat emulsion group received omega-3 fish oil fat emulsion at a dose of 15 mL/kg daily for 14 days. Meanwhile,the ani-mals in control group and model group were intravenously administered with the same volume of saline. the weight and food intake of the mice in each group were assessed daily. Five mice in each group were respectively sacrificed at day 1,day 7,day 14 after intravenous injection. Morphology of gastric mucosa was observed by HE staining and the activities of SOD and MAO in gastric mucosa were measured respectively by xanthine oxida-tion and ultraviolet spectrophotometry methods. Results Compared with the model group,the general status,nutritional status and the injury in stomach mucosa in omega-3 fish oil fat emulsion group were significantly improved. After 14 day′s treatment,the activities of SOD and MAO in gas-tric mucosa of mice in omega-3 fish oil fat emulsion group were significantly increased(P < 0.05)compared with model group. Conclusion omega-3 fish oil fat emulsion has a significant protective effect on the cyclophosphamide induced injury in gastric mucosa of mice,which may be related to the upregulation of MAO and SOD.
