Effects of 5-Aza-CdR on growth of TPC-1 cell line and KLF4 expression

VernacularTitle:5-氮杂-2′-脱氧胞苷对人甲状腺癌 TPC-1细胞生长及 KLF4基因表达的调节作用
Author: Mingchuang LI ; Ruijuan WANG ; Guo CHEN ; Qingsong ZHANG ; Di WU ; Jing LYU ; Yanping HUO ; Detao YIN
Publication Type:Journal Article
Keywords: KLF4 gene; Demethylation; 5-Aza-2′-deoxycytidine; Thyroid cancer
From: Chinese Journal of Endocrine Surgery 2015;(6):480-483,492
CountryChina
Language:Chinese
Abstract: Objective Methylation of anti-oncogene can be demethylated by related drugs which can help the inactivated gene to express again .This study aims to study the effects of the demethylating agent 5-Aza-2′-deoxycytidine on the growth of human thyroid papillary cancer cell line TPC-1 and mRNA and protein expres-sion of KLF4.Methods TPC-1 cells were treated with different concentration of 5-Aza-CdR.MTT was used to detect the influence of 5-Aza-CdR on cell proliferation .RT-PCR was used to detect mRNA and protein expression levels of KLF4.Results After being treated with 5-Aza-CdR for 24 hours, 48 hours, and 72 hours, the growth of TPC-1 cells was inhibited and the inhibition was in time and concentration depended manner .After treatment with 5-Aza-CdR, mRNA and protein expression levels of KLF 4 were increased, and the difference had statistical significance(P<0.05).Conclusion 5-Aza-CdR can inhibit the cell viability of TPC-1 cells through upregulat-ing KLF4 expression , which may provide experimental basis for 5-Aza-CdR in treating thyroid cancer .