Application of EP7-A2 document for investigating interference of hemolysis on total bilirubin detection by diazonium method
10.3969/j.issn.1673-4130.2015.24.007
- VernacularTitle:应用EP7-A2文件探讨溶血对重氮法测定总胆红素的干扰情况
- Author:
Linghua GUAN
;
Jian WU
;
Yuan WANG
;
Hong ZHANG
- Publication Type:Journal Article
- Keywords:
total bilirubin;
diazonium method;
EP7-A2 document;
interference test
- From:
International Journal of Laboratory Medicine
2015;(24):3525-3526,3529
- CountryChina
- Language:Chinese
-
Abstract:
Objective To apply EP7‐A2 document for evaluating the interference of hemolysis the total bilirubin detection in clinic .Methods The interferent was affirmed according to thepaired‐differencetest required by the EP7‐A2 document .The rela‐tion between interferent concentration and interference degree was analyzed by using the dose‐effect test .Results The paired‐difference test results showed that 5 .00 g/L hemoglobin had negative interference effect on two kinds of total bilirubin reagents . But the reagent kit A had little interference degree of hemolysis;The dose‐effect test results showed that hemoglobin produced the linear negative interference effect on the reagent kit A of total bilirubin detection .The linear equation of low value sample was Y=-0 .146X+14 .1 ,r=0 .964 ,which of middle value sample was Y = -0 .546X+92 .24 ,r=0 .947 and which of high value sample was Y= -1 .153X+307 .2 ,r=0 .979 .Conclusion Hemolysis has a negative interference effect on total bilirubin detection in clinic . The total bilirubin value could be corrected by using the hemoglobin concentration of sample;the EP7‐A2 document has certain ap‐plication value in the aspect of analyzing interference and evaluation .
