17-methoxyl-7-hydroxyl-benzofuran chalcone alleviates myocardial ischemia/reperfusion injury via PI3K/Akt signal pathway
10.3969/j.issn.1001-1978.2015.12.020
- VernacularTitle:玉郎伞查尔酮调控 PI3K/Akt 信号通路抗心肌缺血／再灌注损伤的作用及机制研究
- Author:
Feifei XUAN
;
Jianchun HUANG
;
Jingzhi TANG
;
Wansu HUANG
;
Renbin HUANG
- Publication Type:Journal Article
- Keywords:
1 7-methoxyl-7-hydroxyl-benzofuran chal-cone;
regulation;
PI3K/Akt;
apoptosis;
autophagy;
myocardial ischemia /reperfusion injury
- From:
Chinese Pharmacological Bulletin
2015;(12):1730-1734,1735
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of 1 7-me-thoxyl-7-hydroxyl-benzofuran chalcone(YLSC)on my-ocardial ischemia /reperfusion injury(MI /RI)by mod-ulating PI3K/Akt signaling pathway and the possible mechanisms.Methods Male SD rats were randomly
divided into sham group,model group,YLSC group, wortmannin(WM)group and YLSC +WM group(n =8).The rat model of MI /RI was established by ligation of the left anterior descending artery for 30 min fol-lowed by loosening the ligature for 2 h.After reperfu-
sion,blood samples were obtained to determine serum contents of CK-MB,LDH,NO and TNF-α.The pro-tein levels of total (t)-Akt,phosphorylated (p)-Akt and LC3-Ⅱ were detected by Western blot.Caspase-3,Beclin1 and eNOS mRNA expression was evaluated by FQ-PCR.Results YLSC pretreatment greatly re-duced serum levels of CK-MB,LDH and TNF-α,and elevated NO content.It also inhibited the expression of
caspase-3,Beclin1 and LC3-Ⅱ,while enhanced p-Akt and eNOS expression.Conclusion YLSC protects the heart against MI /RI via inhibition of apoptosis and excessive autophagy,in which protective effect is regu-lated by activation of the PI3K/Akt pathway.
