Treatment effect of Tongxinluo combined peripheral blood derived mesenchymal stem cells transplantation on angiogenesis of HIF-1/VEGF pathway and miR-210 in diabetic foot rats
10.3969/j.issn.1001-1978.2015.12.024
- VernacularTitle:通心络联合外周血间充质干细胞移植对糖尿病足大鼠血管新生 HIF-1／VEGF 通路及 miR-210表达的影响
- Author:
Yongying GUO
;
Junfang ZHANG
;
Cunqin JIA
;
Huailin GAO
- Publication Type:Journal Article
- Keywords:
peripheral blood derived mesenchymal stem cells;
transplant;
Tongxinluo;
cilostazol;
diabetic foot rat model;
angiogenesis;
HIF-1 /VEGF pathway
- From:
Chinese Pharmacological Bulletin
2015;(12):1749-1753,1754
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the treatment effect of Tongxinluo(TXL)combined peripheral blood derived mesenchymal stem cells(PB-MSCs)transplantation on angiogenesis of HIF-1 /VEGF pathway and miR-21 0 in diabetic foot(DF)rats.Methods Seventy male Spra-gue-Dawley rats were fed with high-fat diet and given
combined streptozotocin injection to build diabetic rat model.The femoral artery and vein of right lower ex-tremity of modeled rats were ligatured to induce DF model.The normal rats in the same batch copied is-chemia model served as control group.The modeled rats were divided into 6 groups :model group ,TXL
group,Cilostazol group,PB-MSCs group,TXL com-bined PB-MSCs(T-MSCs)group and Cilostazol com-bined PB-MSCs(C-MSCs)group.After the treatment for 28 days,animal gastrocnemius muscle and serum were taken for detection,in which serum was used to detect VEGF-A and HIF-1 α by ELISA,and muscle was used to detect VEGF-A and VEGF-R2 by Western blot,and VEGF-A by RT-PCR.Results Compared with the control group,the expressions of VEGF-A, HIF-1 α,VEGF-R2 and miR-21 0 in the model group were significantly reduced(P <0.01 ).Compared with the model group,the expressions of VEGF-A,VEGF-
R2 and miR-21 0 in each treatment group were signifi-cantly increased(P <0.01 ),where the expressions of VEGF-A,HIF-1 α,VEGF-R2 and miR-21 0 in the T-MSCs were higher than these in the TXL,cilostazol and PB-MSCs group(P <0.05,P <0.01 ).Conclusion TXL combined with PB-MSCs transplantation may be regulated by HIF-1 /VEGF pathway and miR-21 0,pro-moting endothelial cell proliferation,differentiation, and promoting the formation of new blood vessels.
