Effects of tetramethylpyrazine-2′-O-sodium ferulate on swelling of astrocytes and expression of AQP4 after oxygen-glucose deprivation/reoxygenation
10.3969/j.issn.1001-1978.2015.12.008
- VernacularTitle:吡拉格雷钠对氧糖剥夺／复氧后星形胶质细胞水肿及 AQP4表达的影响
- Author:
Shuhong XU
;
Maoshan YIN
;
Yulin WU
- Publication Type:Journal Article
- Keywords:
tetramethylpyrazine-2′-O-sodium ferulate;
astrocyte;
oxygen-glucose deprivation /reoxygenation;
cell volume;
leakage rate of LDH;
cell edema;
Aqua-porin-4
- From:
Chinese Pharmacological Bulletin
2015;(12):1661-1666,1667
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effects of tetrameth-ylpyrazine-2′-O-sodium ferulate (TSF)on the swelling of
astrocytes and the expression of AQP4 after oxygen gl-ucose deprivatio /reoxygenation(OGD /Reox).Methods
Astrocytes were divided into 4 groups:control group, OGD /Reox group,Ozagrel group and TSF group.The effects of TSF on astrocytes were investigated 6,1 2,24 and 48 h after OGD /Reox.The cell injury was assessed by measuring LDH activity and MTT.The expression levels of AQP4 protein of astrocytes were detected u-sing Western blot.Results OGD /Reox induced obvi-ous cell swelling and significant reduction of LDH in astrocytes whereas TSF remarkably attenuated OGD-in-duced astrocyte swelling and LDH reduction (P <0.01 ).The expression levels of AQP4 protein in-creased gradually to the peak at 48 h in OGD /Reox
group(P <0.05 ).AQP4 protein levels in TSF group were much lower compared with OGD /Reox group at each time point (P <0.05).And there was no signifi-cant difference between TSF group and OGD /Reox group(P >0.05 ).Conclusion TSF can attenuate OGD-induced swelling of astrocytes through decreasing the AQP4 expression.
