Correlation study of disintegrin and metalloproteinase with thrombospondin motifs 5 gene single nucleotide polymorphism and osteoarthritis in Beijing
10.3760/cma.j.issn.1007-7480.2015.08.003
- VernacularTitle:含Ⅰ型血小板结合蛋白基序结构的去整合素金属蛋白酶-5基因单核苷酸多态性与北京地区骨关节炎发病的相关性研究
- Author:
Yingjuan CHEN
;
Cibo HUANG
;
Yongjing CHENG
;
Jia HUANG
;
Qian WANG
;
Qian LIU
- Publication Type:Journal Article
- Keywords:
Matrix metalloproteinases,membrane-associated;
Polymorphism,single nucleotide;
Osteoarthritis
- From:
Chinese Journal of Rheumatology
2015;19(8):512-517,后插1
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation of disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)-5 gene single nucleotide polymorphism (SNP) and osteoarthritis (OA) in Beijing.Methods In this case-control study 166 OA patients and 204 normal controls were collected from Beijing.Sorted gene type by SNaPshot technique,and analyzed the difference of allele and genotype frequencies between OA and the controls.The correlation of linkage disequilibrium and haplotype of SNPs with OA was analyzed through Haploview softeware.Results Rs2132824 was the positive site with Bonferroni method after multiple testing correction (x2=0.1 1,P＜0.05),of which allele frequencies had no significant difference between OA and the control (P＞0.05).But in codominant genetic model,rs2132824 CT genotype with OA was inversely proportional to the risk of OA [OR (95％CI) was 0.52 (0.32,0.86),P＜0.05],and TT genotype was directly proportional to the risk of OA [OR(95％CI) was 3.16 (1.55,6.47),P＜0.05],while in recessive genetic model,TT genotype was in direct proportion to the risk of OA [OR (95％CI) was 3.99 (1.99,8.01),P＜0.05].Linkage disequilibrium in SNPs of rs151065,rs229077,rs56153501,rs2830580 and rs58215296 did exist,which made haplotype of rs 151065-rs229077-rs56153501-rs2830580-rs58215296 (G-T-A-C-A) directly proportional to the risk of OA [OR(95％CI) was 1.874(1.019,3.446),P＜0.05].Conclusion ADAMTS-5 gene SNP is associated with OA susceptibility risk in Beijing.Genotype CT of rs2132824 is a low risk factor for OA while TT is a hygh risk factor.Haplotype of G-T-A-C-A is connected with high risk of OA.
