In-cell localization of triptolide
10.3969/j.issn.1671-8348.2015.25.034
- VernacularTitle:雷公藤内酯醇的细胞内定位研究?
- Author:
Ying ZHANG
;
Sha XIAO
;
Wei XU
;
Qingchun DIAO
;
Jin HAO
- Publication Type:Journal Article
- Keywords:
triptolide;
coumarin;
cellular localization
- From:
Chongqing Medicine
2015;(25):3556-3557,3561
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clarify the cellular localization of triptolide and to explore its in-cell action sites.Methods 4-(Bro-momethyl)-7-methoxycoumarin was employed to label triptolide,then labelled triptolide was incubated with human hepatoma carci-noma cells.Subsequently,incubated cells were subjected to stain with fluorescent dye DiI or PI,which were specific to cytoplasmic membrane system and nucleus,respectively.Results Compared with the non-triptolide control,coumarin labelled triptolide shown a light blue fluorescence under UV excitation;Co-localization with DiI showed that triptolide exist in cytoplasm and(or)on cell mem-brane;Co-localization with PI showed that triptolide located in cell nucleus.Moreover,microscopic observation indicated that the fluorescence intensity in nucleus was denser than that in cytoplasm.Conclusion The presnt study demonstrate that triptolide main-ly act in nucleus,followed by acting in cytoplasm and(or)on cell membrane.
