Indirect immunofluorescence and real-time fluorescent PCR for detection of mycoplasma pneumonia in children
10.3969/j.issn.1673-4130.2015.18.007
- VernacularTitle:间接免疫荧光与实时荧光 PC R检测小儿支原体肺炎
- Author:
Jianrong WANG
;
Xu HE
;
Fuguang RAO
- Publication Type:Journal Article
- Keywords:
mycoplasma pneumonia;
children;
indirect immunofluorescence;
real-time fluorescent polymerase chain reac-t io n
- From:
International Journal of Laboratory Medicine
2015;(18):2633-2634,2637
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare efficacy of indirect immunofluorescence (IFA) and real‐time fluorescent polymerase chain re‐action(PCR) in detection of mycoplasma pneumonia in children .Methods A total of 137 children clinically diagnosed as Mycoplas‐ma pneumoniae(MP) infection were selected and divided into groups by age ,including <1 years old group(35 cases) ,1- <5 years old group(69 cases) and 5-15 years old group(33 cases) .Blood specimen and throat swabs were collected and detected by using IFA and real‐time fluorescent PCR .At the same time ,all of the selected children were treated with conventional therapy ,according to total effective rate ,positive coincidence rates of the two methods were statistically analysed by age .Results The positive coinci‐dence rates in children with MP infection <1 years old and 1- <5 years old detected by using real‐time fluorescent PCR were high‐er than that detected by using IFA ,while among children 5-15 years old ,the positive coincidence rate was higher detected by using IFA compared with that detected by using real‐time fluorescent PCR ,all had statistically significant differences (P<0 .05) .The o‐verall positive coincidence rates of the two methods were not significantly different(P>0 .05) .Conclusion IFA and real‐time fluo‐rescent PCR both could be used as effective methods for detecting MP ,but there are some differences of detective efficacy between the two methods in each age group .Therefore ,it is suggested that for children under 5 years old real‐time fluorescent PCR might be selected ,for children aged 5 years old and over IFA might be selected ,in order to improve the detection accuracy and provide better guidance to clinical medication .
