Changes of interleukin-17A and interleukin-22 in the lung of cigarette smoke exposed mice and the effect of N-acetylcysteine
- VernacularTitle:N-乙酰半胱氨酸对香烟暴露小鼠肺组织中 IL-17A 及 IL-22的影响
- Author:
Di FENG
;
Xin GUO
;
Xiaodong MEI
- Publication Type:Journal Article
- Keywords:
cigarette smoking;
N-acetylcysteine;
interleukin-17A;
interleukin-22
- From:
Acta Universitatis Medicinalis Anhui
2015;(11):1579-1582,1583
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of interleukin-17A(IL-17A)and interleukin-22(IL-22)in the lung of cigarette smoke exposed mice and the effect of smoking cessation and N -acetylcysteine (NAC )treatment . Methods BALB /c mice in experimental groups were exposed to cigarette smoke.Then the smoke-exposure was stopped and mice were treated with NAC gavage.The mice were executed 1,2,and 3 months after smoking cessa-tion.Lung tissue sample and bronchoalveolar lavage fluid(BALF)were collected.HE staining was used to observe the pathologic changes of the lung and ELISA was used to measure the level of IL-17A and IL-22.Results Con-siderable emphysematous changes was found in the lung of mice exposed to cigarette smoke.Compared with the controls,the level of IL-17A and IL-22 elevated remarkably in pulmonary tissue and BALF after smoking exposure and declined gradually after smoking cessation.Additional NAC gavage treatment enhanced the decline tendency. Conclusion IL-17A and IL-22 might play a complex role in the chronic inflammatory changes of lung in mice ex-posed to cigarette smoke.
