Application of real-time fluorescence isothermal RNA amplification assay in the determination of Mycobacterium tuberculosis in pleural effusion
10.3969/j.issn.1673-4130.2015.18.033
- VernacularTitle:实时荧光核酸恒温扩增检测技术在胸腔积液结核分枝杆菌检测中的应用
- Author:
Song CHENG
;
Chengyong LIU
;
Dongqing ZHOU
;
Yuanpei HOU
- Publication Type:Journal Article
- Keywords:
pleurisy;
Mycobacterium tuberculosis;
nucleic acid amplification techniques
- From:
International Journal of Laboratory Medicine
2015;(18):2697-2698,2701
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate clinical value of utilization of real‐time fluorescence isothermal RNA amplification assay (SAT)for detecting Mycobacterium tuberculosis(MTB) in pleural effusion samples of patients with tuberculous pleurisy .Methods 90 hydrothorax specimens of patients with tuberculous pleurisy and 52 hydrothorax specimens of patients with non‐tuberculous pleurisy were selected ,and MTB was detected by using Roche culturing method ,SAT and TB DNA fluorescence quantitative expan‐sion method respectively .The sensitivity ,specificity and clinical value of SAT for detecting MTB were analysed .Results Taking Roche culturing method as the standard ,the sensitivity ,specificity and Youden index of SAT for detecting MTB were 90 .9% , 72 .1% and 0 .630 respectively .In patients clinically diagnosed with tuberculous pleurisy ,the positive detection rate of hydrothorax specimens detected by using Roche culturing method and SAT were 24 .4% and 43 .3% respectively ,and had statistically significant differences(χ2 =7 .166 3 ,P=0 .001 4) .Conclusion SAT is a rapid ,sensitive and specific method for the detection of MTB in pleu‐ral effusion samples ,which could improve the detection rate of MTB and decrease incidence of missed diagnosis and misdiagnosis .
