Investigation and Analysis of Reference Intervals of Cystatin C andβ2-Microglobulin
10.3969/j.issn.1671-7414.2015.04.008
- VernacularTitle:半胱氨酸蛋白酶抑制剂 C和β2微球蛋白参考区间的调查与分析
- Author:
Kun ZHONG
;
Wei WANG
;
Falin HE
;
Yan ZHANG
;
Yan ZHAO
;
Guohua LI
;
Zhiguo WANG
- Publication Type:Journal Article
- Keywords:
Cystatin C;
β2-microglobulin;
reference interval
- From:
Journal of Modern Laboratory Medicine
2015;(4):32-34,38
- CountryChina
- Language:Chinese
-
Abstract:
Objective To know the current application status of reference interval of cystatin C andβ2-microglobulin in medi-cal institutions of China.Methods Every laboratory which attended 2014 specific protein external quality assessment pro-gram of national center of clinical laboratories has been received an internet questionnaire to survey reference intervals of cystatin C andβ2-microglobulin.After eliminating outliers the trimmed data was statistical analyzed by Microsoft Excel 2007 and SPSS 13.0.The resources of RIs,traditional statistical analysis,normalized analysis,comparison between overall median and lower and upper limits of different measurement system,and verification status of RIs were included.Results 106 and 127 medical laboratories submitted the questionnaire and the number of province with submission of RIs for the two renal function measurement items were 24 and 29,respectively.Less than 5 laboratories grouped their RIs according to age and/or gender.Approximately 75% of laboratories had been using the RIs referring to reagent manufacturers’instructions.All me-dians of upper or lower limits of analytes were as same as the Mode values of the same group.The 2.5th percentiles had tre-mendous differences compared with 97.5th percentiles.More than half of laboratories did not verify their RIs beforeusing them.The overall medians of lower and upper limits of RIs were (in serum):Cystatin C (mg/L)0.55 and 1.09;β2-micro-globulin (μg/ml)1.00 and 2.80.Conclusion The RIs of cystatin C and β2-microglobulin used in medical institution had some differences between each other,and there were too many kinds of reagents and instruments which made different meas-urement systems.The consistent and standardized reference intervals should be established in a short time.The RI for cysta-tin C was 0.55~1.09 mg/L,forβ2-microglobulin was 1.00~2.80 μg/ml considered by this study.
