Method evaluation of plasmodium DNA template extraction from dried blood on filter paper
10.3969/j.issn.1673-4130.2015.20.040
- VernacularTitle:滤纸干血斑制备疟原虫DNA 模板的方法评价
- Author:
Honge ZHU
;
Bin TIAN
- Publication Type:Journal Article
- Keywords:
plasmodium spp;
DNA;
polymerase chain reaction;
dried blood spots
- From:
International Journal of Laboratory Medicine
2015;(20):3016-3017,3029
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the method of plasmodium DNA template extraction from dried blood on filter paper . Methods Collectedand tested 47 blood samples of suspected imported malaria patients ,the DNA was extracted and tested by using conventional procedures and PCR ,then count and analyze test results of matched design .Results The Kappa value was 0 .492 when compared filter paper dried blood spots with thick and thin blood smear test results ,and the Kappa value was 0 .686 when com‐pared with whole blood sample .Using the results of thick and thin blood smear and whole blood samples as the gold standard to e‐valuate the sensitivity and specificity of filter paper dried blood spot method respectively ,the sensitivity was 75 .0% ,71 .4% and specificity was 78 .9% ,100 .0% .Conclusion Collected blood samples using filter paper can′t get good results in PCR test ,still needs further research .
