Effects of Kunmu decoction on proliferation and apoptosis of fibroblast-like synoviocytes in rheumatoid arthritis
10.3969/j.issn.1006-5725.2015.17.009
- VernacularTitle:昆母汤对类风湿关节炎滑膜细胞增殖及凋亡的影响
- Author:
Xiumin CHEN
;
Changsong LIN
;
Qingping LIU
;
Qiang XU
;
Tong GUAN
;
Jifan CHEN
;
Fengzhen LIU
;
Ying WU
- Publication Type:Journal Article
- Keywords:
Arthritis,rheumatoid;
Kunmu decoction;
Fibroblast-like synoviocytes;
Proliferation;
Apoptosis
- From:
The Journal of Practical Medicine
2015;(17):2793-2795,2796
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of alcohol extract of Kunmu decoction on proliferation and apoptosis of rheumatoid arthritis fibroblast-like synoviocytes (RA-FLS). Methods Synovial tissues were obtained from patients with active RA received joint replacement or arthroscopy. The surface antigen and the amount of apoptotic cells were determined by flow cytometry. The inhibitive effect was detected by MTT assay. Results The CD90+surface antigen of synoviocytes was (94.78 ± 0.98)%. The inhibitive effect on the proliferation in all treatment groups were in a time-and dose-dependent manner. The apoptosis rate was increased in a dose-dependent manner among all dosage alcohol extract groups. Conclusion Kunmu decoction might inhibit proliferation and induce apoptosis of RA-FLS.
